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immunoblot analysis wes automated western blotting system  (Protein Simple Inc)
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Protein Simple Inc immunoblot analysis wes automated western blotting system
Profiling of photosynthesis-related machinery. A, Expression of selected genes involved in photosynthesis. Log2-fold changes in expression >1 were normalized between 0 and 1 and used for heat map generation, and changes with P ≤ 0.01 were considered as statistically significant (P values are given in Supplemental Dataset 2). Red indicates up-regulation of expression and blue a corresponding down-regulation. Descriptions of the genes and expression values can be found in Supplemental Dataset 2. B, A representative digital <t>immunoblot</t> for PsbA (D1), cytochrome b6f, ATP synthase, and RbcLII proteins. N+, 72 h under N-replete conditions; N−, 72 h of N deprivation; NR, 72 h after NR. C, Profiles of molecular weights of protein bands shown in B. D, Abundance of proteins from B under different N conditions. Each data point represents the average of three biological replicates. Values represent means ± sd (n = 3). E, SDS-PAGE gel of total proteins loading control stained by Coomassie blue.
Immunoblot Analysis Wes Automated Western Blotting System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoblot analysis wes automated western blotting system - by Bioz Stars, 2026-07
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Profiling of photosynthesis-related machinery. A, Expression of selected genes involved in photosynthesis. Log2-fold changes in expression >1 were normalized between 0 and 1 and used for heat map generation, and changes with P ≤ 0.01 were considered as statistically significant (P values are given in Supplemental Dataset 2). Red indicates up-regulation of expression and blue a corresponding down-regulation. Descriptions of the genes and expression values can be found in Supplemental Dataset 2. B, A representative digital immunoblot for PsbA (D1), cytochrome b6f, ATP synthase, and RbcLII proteins. N+, 72 h under N-replete conditions; N−, 72 h of N deprivation; NR, 72 h after NR. C, Profiles of molecular weights of protein bands shown in B. D, Abundance of proteins from B under different N conditions. Each data point represents the average of three biological replicates. Values represent means ± sd (n = 3). E, SDS-PAGE gel of total proteins loading control stained by Coomassie blue.

Journal: Plant Physiology

Article Title: The Microalga Nannochloropsis during Transition from Quiescence to Autotrophy in Response to Nitrogen Availability 1 [OPEN]

doi: 10.1104/pp.19.00854

Figure Lengend Snippet: Profiling of photosynthesis-related machinery. A, Expression of selected genes involved in photosynthesis. Log2-fold changes in expression >1 were normalized between 0 and 1 and used for heat map generation, and changes with P ≤ 0.01 were considered as statistically significant (P values are given in Supplemental Dataset 2). Red indicates up-regulation of expression and blue a corresponding down-regulation. Descriptions of the genes and expression values can be found in Supplemental Dataset 2. B, A representative digital immunoblot for PsbA (D1), cytochrome b6f, ATP synthase, and RbcLII proteins. N+, 72 h under N-replete conditions; N−, 72 h of N deprivation; NR, 72 h after NR. C, Profiles of molecular weights of protein bands shown in B. D, Abundance of proteins from B under different N conditions. Each data point represents the average of three biological replicates. Values represent means ± sd (n = 3). E, SDS-PAGE gel of total proteins loading control stained by Coomassie blue.

Article Snippet: For capillary immunoblot analysis Wes automated western blotting system (ProteinSimple) was used according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, SDS Page, Staining